Fig. 2

Histological analysis of RV tissue in the 4 groups of rats. a Representative image of the RV cryosections stained with H&E (× 400). Nuclei were stained dark purple, the cytoplasm was stained red, the scale bar is 50 µm. b Representative images of the RV WGA immunofluorescence detection (× 400), the scale bar is 50 µm. c Representative images of the RV cryosections stained with Masson’s trichrome (× 200). Collagen fibres are stained blue, nuclei are stained dark purple, and cytoplasm is stained red/pink, the scale bar is 100 µm. d Quantitative analysis of the RV cardiomyocyte cross-sectional area that was WGA-stained (µm²). e Quantitative analysis of the average CVF from the Masson’s trichrome-stained section in the RV (%). WGA wheat germ agglutinin, CVF collagen volume fraction. N = 5 per group. The horizontal lines show the mean ± SEM. One-way ANOVA. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001