Fig. 6

Effects of MLKKL knockout in streptozocin (STZ)-induced diabetic hearts. a Adult cardiomyocytes isolated from MLKL knockout (MLKL-KO) and wild-type mice (WT) were incubated with high glucose or Mannitol (25 mmol/L) for 24 h. LDH was measured in culture medium. b–g MLKL-KO and WT mice were rendered diabetic by STZ injection. b and c Echocardiographic analysis for myocardial function. d Necrotic cell death in the heart was determined by Evans blue staining assay. Upper panel: representative micro-photograph for necrotic cells in the heart; Bottom panel: quantification of necrotic cell death. e and f Biomarkers of myocardial injury: serum CK-MB (e) and cardiac troponin I (f). g Myocardial fibrosis was assessed by collagen deposition in the heart. Upper panel: representative micro-photograph for collagen deposition in the heart (red); Bottom panel: quantification of collagen deposition. Data are mean ± SD, n = 5–6. *P < 0.05 versus Sham + WT and ^†P < 0.05 versus STZ + WT (ANOVA)