Fig. 5
From: MLKL-mediated necroptosis is a target for cardiac protection in mouse models of type-1 diabetes
Effects of GSK’872 in streptozocin (STZ)-induced diabetic hearts. Wild-type mice were rendered diabetic by STZ injection and after the induction of diabetes, GSK’872 was administrated for a total of 2 months. a The levels of phosphorylated MLKL (p-MLKL) were analyzed by western blot in the heart. Upper panel: a representative western blot from 2 out of 6 different hearts in each group for p-MLKL and total MLKL; Bottom panel: quantification of p-MLKL relative to total MLKL. b Myocardial fibrosis was assessed by collagen deposition in the heart. Upper panel: representative micro-photograph for collagen deposition in the heart (red); Bottom panel: quantification of collagen deposition. c Necrotic cell death in the heart was determined by Evans blue staining assay. Upper panel: representative micro-photograph for necrotic cells in the heart; Bottom panel: quantification of necrotic cell death. d and e Biomarkers of myocardial injury: serum cardiac troponin I (e) and CK-MB (d) and. f and g Echocardiographic analysis for myocardial function. Data are mean ± SD, n = 5–6. * P < 0.05 versus Sham + Vehicle and †P < 0.05 versus STZ + Vehicle (ANOVA)