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Fig. 3 | Cardiovascular Diabetology

Fig. 3

From: MLKL-mediated necroptosis is a target for cardiac protection in mouse models of type-1 diabetes

Fig. 3

Characterization of cardiomyocyte necroptosis in the heart. a and e Necrotic cell death was assessed by Evans blue staining assay. Representative micro-photographs show Evans blue stained necrotic cell death (red) in hearts of STZ-injected mice and Akita mice but not in Sham animals. Nuclei were stained blue by Hoechst33342. b and f Quantification of Evans blue stained necrotic cell death in the heart. c and g Serum cardiac troponin I. d and h MLKL oligomerization. Representative western blot from 4 different hearts in each group shows the presence of MLKL oligomerization in STZ-injected and Akita but not Sham mouse hearts. i Confocal microscopy. Representative micro-photograph indicates that Evans blue stained necrotic cells (red) are presented with phosphorylated MLKL (p-MLKL, blue), and that punctate staining of p-MLKL co-localizes with WGA-FITC (cyan). Data are mean ± SD, n = 5–6. * P < 0.05 versus Sham or WT (t test)

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