Fig. 1

Phosphorylation of RIPK3 and MLKL. a Adult mouse cardiomyocytes were incubated with high glucose or mannitol (25 mmol/L) for 24 h. The levels of phosphorylated RIPK3 (p-RIPK3) and MLKL (p-MLKL) were determined by western blot analysis. Left panel: representative western blots for p-RIPK3, p-MLKL and their total proteins as well as GAPDH; Right two panels: quantification of p-RIPK3 and p-MLKL relative to their total proteins, respectively. b–d The levels of p-RIPK3 and p-MLKL in hearts of streptozotocin (STZ)-injected mice (b), Akita mice (c) and monkeys (d) were analyzed by western blot. Left panel: representative western blots from 3 out of 5–6 different hearts in each group for p-RIPK3 and p-MLKL, total RIPK3, total MLKL and GAPDH; Right two panels: quantification of p-RIPK3 and p-MLKL (WT: wild-type). Data are mean ± SD, n = 5–6. * P < 0.05 versus Mannitol, Sham or WT (t test)