Fig. 6

AZD5069, under high fat diet conditions, prevents the accumulation of lipid droplets as well as activated neutrophils in liver. Livers from mice at 16 weeks under each of the study diets were evaluated for the presence of lipid droplets and activated neutrophils by immunofluorescence microscopy. A Representative images of livers stained with the lipid indicator LipidTox, the neutrophil marker Ly6G, and the activated inflammasome marker IL-18. B–D Quantifications of marker content or cell number per fixed tissue area are shown. HF + AZD displays the accumulation of lipid droplets and IL-18 + cells in livers of mice on the HF + AZD diet is intermediate to those of livers from mice on the CN and HF diets, whereas, the accumulation of Ly6G + cells (neutrophils) is statistically-indistinguishable from that of livers of mice in the CN diet. In graphs B–D, the bars show the median of the measurements in liver sections of n = 5–9 mice of each diet arm and the error bars show the SD. Significant differences were ascertained by one-way ANOVA or Kruskal-Wallis test followed by Bonferroni post-test. Significant differences are shown where *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001