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Fig. 1 | Cardiovascular Diabetology

Fig. 1

From: Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis

Fig. 1

Empagliflozin reduces I/R injury-induced cardiac microvascular damage. Mice were subjected to 45 min of ischemia followed by two hours of reperfusion to induce cardiac I/R injury. Empagliflozin (EMPA, 10 mg/kg/d) was administered for seven days before myocardial I/R injury. A Electron microscopy was used to detect structural alterations in cardiac microvessels. Yellow arrows indicate the thickened wall and narrowed lumen. B, C. Immunofluorescence was used to detect fibrin accumulation in microvessels. D, E Proteins were isolated from reperfused heart and the expression of fibrin was determined by western blots. F, G Heart samples were collected after I/R injury for immunohistochemical analysis of ICAM1. H –J Total mRNA was isolated from reperfused hearts, and the mRNA levels of IL-6, MCP-1 and TNFα were determined using quantitative PCR. Data are shown as mean ± SEM, n = 6 mice per group. *p < 0.05

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