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Table 1 List of Western blot antibodies

From: Liraglutide preserves CD34+ stem cells from dysfunction Induced by high glucose exposure

 

Code (purchased from)

Source

Dilution

Primary antibody

 GLP-1R (D-6)

Sc-390774

(Santa Cruz Biotecnology)

Mouse

1:200

 Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204)

#4370

(Cell signaling)

Rabbit

1:2000

 p44/42 MAPK (ERK1/2)

#9102

(Cell signaling)

Rabbit

1:1000

 Phospho-AKT (Ser473)

#9271

(Cell signaling)

Rabbit

1:1000

Secondary antibody

 ECL Anti-rabbit IgG

NA9340

(Amersham Biosciences)

donkey

1:10000

 ECL Anti-mouse IgG

NA9310

(Amersham Biosciences)

sheep

1:5000