Fig. 9

LIRA partially recovered HG-damaged CD34⁺ HSPC functions. A Proliferation curve of HG-damaged CD34⁺ HSPCs after 10 days stimulation with 100 nM LIRA (***p ≤ 0.001 HG vs NG; two-way ANOVA). B Analysis by flow cytometry of CXCR4 expression level in HG-damaged CD34⁺ HSPCs after 10 days of LIRA treatment (***p ≤ 0.001 HG vs NG; * p ≤ 0.05 HG 100 LIRA vs HG; one-way ANOVA). C Migration of HG-damaged CD34⁺ HSPCs after 10 days of LIRA treatment (**p ≤ 0.01 HG vs NG; ** p ≤ 0.01 HG 100 LIRA vs HG; one-way ANOVA). D Quantification of intracellular ROS level by flow cytometry in HG-damaged CD34⁺ HSPCs after 10 days of LIRA treatment (*p ≤ 0.05 HG vs NG; * p ≤ 0.05 HG 100 LIRA vs HG; one-way ANOVA). HG = high glucose, LIRA = liraglutide, NG = normal glucose