Skip to main content
Fig. 7 | Cardiovascular Diabetology

Fig. 7

From: Liraglutide preserves CD34+ stem cells from dysfunction Induced by high glucose exposure

Fig. 7

Intracellular pathway cross-talk after GLP-1R1 stimulation with 100 nM LIRA. A Representative immunoblots of ERK1/2 and C AKT phosphorylation in CD34+ HSPCs treated with 100 nM LIRA for 5, 10 and 15 min and after the addition of MEK1/2 and PI3K selective pathway inhibitors, PD (E) and WT (G) respectively. GAPDH was used as loading control. B, D, F, H Immunoblotting quantification presented as arbitrary units after normalization to the GAPDH protein levels of three independent experiments. AKT = protein kinase B, ERK1/2 = extracellular signal-regulated kinases 1 and 2 GAPDH = Glyceraldehyde 3-phosphate dehydrogenase, LIRA = liraglutide, p-AKT = phospho-AKT, p-ERK1/2 = phospho-ERK1/2, PD = PD 98,059, WT = wortmannin

Back to article page