Fig. 4

LIRA treatment prevented CD34⁺ HSPC proliferation, CXCR4/SDF-1α axis impairment and ROS accumulation caused by 20 days HG exposure. A Proliferation curve of CD34⁺ HSPC amplified in NG, HG ± 50 nM and 100 nM LIRA conditions (**p ≤ 0.01 HG vs NG; §§ p ≤ 0.01 HG 100 LIRA vs HG; two-way ANOVA). B Doubling time of CD34⁺ HSPCs cultured in NG, HG ± 50 nM and 100 nM LIRA (**p ≤ 0.01 HG vs NG; **p ≤ 0.01 HG 100 LIRA vs HG; one- way ANOVA). C CD34⁺ HSPC migration towards SDF-1α (50 ng/mL) after NG, HG ± 50 nM and 100 nM LIRA treatment (*p ≤ 0.05 HG vs NG; *p ≤ 0.05 HG 50 LIRA vs HG; ***p ≤ 0.001 HG 100 LIRA vs HG; one-way ANOVA). D Analysis by flow cytometry of CXCR4 expression in NG, HG ± 50 nM and 100 nM LIRA treated CD34⁺ HSPCs (***p ≤ 0.001 HG vs NG; ** p ≤ 0.01 HG 100 LIRA vs HG; one-way ANOVA). E Representative dot plot of CXCR4 cytofluorimetric analysis. HG = high glucose, LIRA = liraglutide, NG = normal glucose