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Fig. 2 | Cardiovascular Diabetology

Fig. 2

From: Liraglutide preserves CD34+ stem cells from dysfunction Induced by high glucose exposure

Fig. 2

GLP-1R expression in CD34+ HSPCs. mRNA expression was assessed by RT-qPCR in 3 different CD34+ HSPC biological replicates. An exon-exon spanning reverse primer was used to exclude any amplification of possible contaminating DNA; capan-1 cells were used as positive control (A). The identity of RT-qPCR products was determined by melting curve (B), agarose gel run (347 bp) (C) and finally by Sanger sequencing (D). CD34+ HSPC lysates (40 μg) deriving from three biological replicates were immunoblotted in order to evaluate GLP-1R protein expression. Capan-1 cell lysate (20 μg) was used as positive control (E). S1, S2, S3 = samples 1, 2, 3

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