Fig. 6

miR-30 regulates a network of genes that are involved in fatty acid biosynthesis and metabolism pathways. A KEGG Pathway analysis (DIANA-miRPath v3.0 with Tarbase v7.0) of human miR-30d-5p and miR-30e-5p target genes reveals a significant enrichment in fatty acid biosynthesis and fatty acid metabolism genes. Pathway analysis in mouse is shown in Additional File 1: Fig. S7. B Top; schematic of experimental approach to assess the function of miR-30 in cultured ECs. Bottom; qRT-PCR of miR-30d and miR-30e in cells transfected with miR-30e mimic (left) or a miR-30 family LNA inhibitor (right) compared to their respective controls. Data is relative to the unstimulated control for each independent experiment. * and ** indicate p < 0.05 and p < 0.01, respectively, for the specified comparison using ANOVA with Holm-Sidak multiple comparisons test. C Heat map of changes in gene expression of putative miR-30 target genes in fatty acid biosynthesis/metabolism pathways by qRT-PCR in ECs over-expressing miR-30e under control or palmitate-stimulated conditions. The mean of multiple independent experiments is indicated. D Representative western blots of FADS1, ELOVL5 and β-actin loading control in ECs over-expressing miR-30e with or without palmitate treatment. Densitometry is included below from n = 3 independent experiments (mean ± SEM). E Heat map of changes in gene expression of putative miR-30 target genes by qRT-PCR in ECs transfected with miR-30 family LNA inhibitor under control or palmitate-stimulated conditions. The mean of multiple independent experiments is indicated. F Representative western blots of FADS1, ELOVL5 and β-actin loading control in ECs transfected with miR-30 family inhibitor. Densitometry is included below from n = 3 independent experiments (mean ± SEM). See Additional File 1: Fig. S8 for individual data points and statistical analysis of data presented as heatmaps in (C) and (E). All data in the figure depict mean ± SEM