Fig. 4

miR-30d-5p and miR-30e-5p are upregulated in the cardiac endothelium in T2D mice. A qRT-PCR of miR-30e-5p in the specified tissues and peripheral blood mononuclear cells in db/db mice and db/ + controls at 14 weeks of age. Data is relative to the mean of the control group for each tissue sample. * and *** indicate p < 0.05 and p < 0.001, respectively, for db/db vs. db/ + in the specified tissue using an unpaired t-test. B Confirmation of EC enrichment of CD31⁺ cells isolated from the heart in db/db and db/ + controls at 14 weeks of age by qRT-PCR of Pecam1 mRNA. Data is relative to the mean of the CD31⁻ control group. * indicates p < 0.05 for the specified comparisons using ANOVA with Holm-Sidak multiple comparisons test. NS = not significant. C miR-30d-5p and miR-30e-5p are specifically upregulated in the CD31⁺ endothelium of the heart of db/db mice at 14 weeks of age compared to db/ + controls. Data is relative to the mean of the CD31⁻ control group. ** indicates p < 0.01 for the specified comparisons using ANOVA with Holm-Sidak multiple comparisons test. NS = not significant. D Confocal imaging of miRNAscope with a miR-30e-5p probe in left ventricles of db/db and control db/ + mice at 14 weeks. A ‘no probe’ control is shown to the right. Arrows indicate regions of expression that are presumed to be endothelial based on cell morphology and anatomy. Representative images are shown. Scale bar = 50 μm. All data in the figure depict mean ± SEM