Fig. 2

Circulating extracellular vesicles are increased in size and concentration in T2D mice. A Nanoparticle tracking analysis (NTA) of EV concentration binned by size from samples isolated from an equal volume of plasma from db/db and db/ + controls at 8 and 14 weeks. n = 4–5 samples per group. B Quantification of EV concentration across all size bins and mode particle size in 8- and 14-week db/db and db/ + control mice. *, ** and *** indicate p < 0.05, p < 0.01 and p < 0.001, respectively, for db/db vs. db/ + at the specified timepoint using an unpaired t-test. C Western blotting of EV markers (CD63, CD81 and TSG101) and a non-EV marker (Calnexin) in EV samples and a total plasma control from 3 db/db and 3 db/ + control mice at 14 weeks. The position of molecular weight markers is indicated to the left and arrows to the right indicate the correct band. D Cryo-transmission scanning electron microscopy of EV samples from db/db and db/ + controls at 14 weeks. A representative image is shown. Arrows indicate examples of EV structures. Scale bar = 200 nm. All data in the figure depict mean ± SEM