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Fig. 6 | Cardiovascular Diabetology

Fig. 6

From: Asprosin induces vascular endothelial-to-mesenchymal transition in diabetic lower extremity peripheral artery disease

Fig. 6

Asprosin induces EndMT in HUVECs. A qPCR analyses of mRNA levels for genes related to EndMT in HUVECs treated with vehicle control, 50 μmol/l asprosin, 10 μmol/l SB431542 (n = 4 for each group). The mRNA expression is normalized to ACTB (β-actin). B, C Representative western blots of indicated molecules in HUVECs. The quantitative results are shown in C (n = 6). Protein levels were normalized to those of β-actin. D Representative immunofluorescent chemical staining of CD31 and α-SMA in HUVECs. Scale bar, 100 μm. Results are expressed as mean ± s.d in A and C. *P < 0.05, ***P < 0.001, compared with control group; #P < 0.05, ###P < 0.001, compared with asprosin group, by one-way ANOVA

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Cardiovascular Diabetology

ISSN: 1475-2840