Fig. 4

Mice with diabetes exhibit systemic monocytosis. A Flow cytometry contour plots display gating strategy for circulating leukocytes in whole blood (summarised in B and C). For full gating strategy see Additional file 1: Figure S2. B–C Proportions of circulating myeloid leukocytes and lymphocytes in ND (n = 7) vs. DM (n = 19) mice. Monocytes (Monos.), Ly6C^hi monocytes (Ly6C^hi), Neutrophils (Neuts.) and Ly6C^lo monocytes (Ly6C^lo). Data is displayed as mean ± SEM. *P < 0.05 (Student’s unpaired t-test)