Fig. 3
From: Glycated ACE2 receptor in diabetes: open door for SARS-COV-2 entry in cardiomyocyte
Glycosylated ACE2 and TMPRSS2 protein levels. a–c, Representative images and bar graph of Western blotting analysis (n = 4) of glycosylated (Glyc) ACE2, total ACE2 and Glyc/total ACE2 ratio in heart tissue from patients without diabetes (Non-DM) Non-COVID-19 (n = 43) versus heart tissue from patients with diabetes (DM) Non-COVID-19 (n = 7), (p = 0.03276 and p = 0.047, respectively) and Non-DM COVID-19 (n = 17) versus DM COVID-19 (n = 30), (p = 0.002391 and p = 0.0025, respectively). Shown as mean ± SD. Statistical test: Student’s t-test. Bonferroni correction was used to make pairwise comparisons. d, e Representative images and bar graph of Western blotting analysis (n = 3) of TMPRSS2 in Non-DM Non-COVID-19 (n = 43) versus DM Non-COVID-19 (n = 7), (p = 0.0344) and Non-DM COVID-19 (n = 17) versus DM COVID-19 (n = 30), (p = 0.001) heart samples. Protein expression was determined by ImageJ 1.52n software and quantified using α-tubulin or GAPDH. Values are expressed as arbitrary units (A.U.). Shown as mean ± SD. Statistical test: See (a–c). *p < 0.05 vs. non-DM (Non-COVID-19); §p < 0.01 vs. non-DM (COVID-19). ACE2 angiotensin-converting enzyme 2, TMPRSS2 transmembrane protease serine 2, GAPDH glyceraldehyde 3-phosphate dehydrogenase