Fig. 3

Ang II causes a redox-sensitive up-regulation of SGLT1 and 2 promoting their own expression in ECs. ECs are incubated with either (a, b) N-acetyl cysteine (NAC, an antioxidant, 1 mM) for 2 h, VAS-2870 (VAS, a NADPH oxidase inhibitor, 1 µM), indomethacin (INDO, a cyclooxygenase inhibitor, 30 µM) or myxothiazol (0.5 µM) + KCN (1 µM) + rotenone (1 µM; MKR, mitochondrial respiratory chain inhibitors) for 30 min, and (c, d) sotagliflozin (SOTA, 100 nM) or empagliflozin (EMPA, 100 nM) for 30 min before the addition of Ang II and the subsequent assessment of the expression level of SGLT1 and SGLT2 by Western blot analysis. Results are shown as representative immunoblots (upper panels) and corresponding cumulative data (lower panels). Data are expressed as mean ± SEM of n = 3. ^*P < 0.05 vs. control and ^#P < 0.05 vs. Ang II