Fig. 4
Activation of pro-survival and pro-angiogenic factors in the db/db-ChAT-tg mice. a–d, f Representative blots and quantitative bar graphs with scatter plots showing the protein expression of M2AChR (a), p-Akt and Akt (b), HIF-1α (c), GLUT-4 (d), VEGF-A (f) and TGF-β1 (g) in the ND, db/db and db/db-ChAT-tg mice. Data are presented as mean ± SEM. A non-parametric Kruskal–Wallis test with Bunn’s test was performed. e The cardiac glucose content was increased by 1.94-fold in the db/db-ChAT-tg mice in comparison to the db/db mice. Data are presented as mean ± SEM. One-way ANOVA with Tukey’s test for multiple comparisons was performed. *p < 0.05, **p < 0.01, ***p < 0.001 vs age-matched ND mice; ##p < 0.01,###p < 0.001; ####p < 0.0001 vs age-matched db/db mice; The number of samples per group is indicated in the figure