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Fig. 4 | Cardiovascular Diabetology

Fig. 4

From: Insulin directly stimulates mitochondrial glucose oxidation in the heart

Fig. 4

Inhibition of mitochondrial Akt abrogates insulin stimulation of PDH. Hearts from the second series of perfusions with the pharmacological inhibitors (as shown in Fig. 3) were homogenized and fractionated using differential centrifugation to isolate mitochondrial and cytosolic portions. a Western blots of Western blots of GLUT1, GLUT4, Akt, PKC-δ, GSK-3β and PDH and their correspondent phosphorylated serine and tyrosine groups. Prohibitin and α-tubulin were employed as loading controls for mitochondrial and cytosolic proteins, respectively. Densitometric analysis of phosphorylated/total levels of b GLUT1, c GLUT4, d Akt, e GSK-3β, f PKC-δ and g PDH in the mitochondria and in the cytosol. Individual values for each group are presented as a scattered plot along with its mean ± SEM (n = 6 for each experimental group). Data were analyzed using Two-way ANOVA followed by Bonferroni post hoc test for multiple comparisons (*p < 0.05)

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