Fig. 6
Changes in mRNA abundance of genes related to NO-signaling pathway. a–c mRNA expressions of VEGF, eNOS and ET-1 in Zucker rat myocardium. GAPDH gene was used as an internal control. The ΔΔcT values are shown as the mean ± SEM. d–h The effects of LIRA treatment on expression of VEGF, total-eNOS, ET-1 and GLP-1R in the left ventricle. d Protein expression levels of VEGF, total-eNOS, ET-1 and GLP-1R detected by Western blotting. β-actin was used as an internal control. Bands are representative of five separate experiments. e–h Relative density values of VEGF, total-eNOS, ET-1 and GLP-1R. N = 5–6 rats per group. The significance of group differences was determined by ANOVA for factors of genotype (G), drug treatment (T) and their interaction term (I)