Fig. 4

The effects of LIRA treatment on expression of CD-68, NF-κB, TGF-β1 and IL-1β in the left ventricle. a Protein expression levels of CD-68, NF-κB, TGF-β1 and IL-1β detected by Western blotting. β-actin was used as an internal control. Bands are representative of four separate experiments. b–e Relative density values of CD-68, NF-κB, TGF-β1 and IL-1β. f mRNA expression of Vcam-1 in Zucker rat myocardium. GAPDH gene was used as an internal control. The ΔΔcT values are shown as the mean ± SEM. N = 5–6 rats per group. The significance of group differences was determined by ANOVA for factors of genotype (G), drug treatment (T) and their interaction term (I)