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Fig. 2 | Cardiovascular Diabetology

Fig. 2

From: Luseogliflozin attenuates neointimal hyperplasia after wire injury in high-fat diet-fed mice via inhibition of perivascular adipose tissue remodeling

Fig. 2

Effect of luseogliflozin on PVAT remodeling and its association with vascular injury in HFD-fed mice. PVAT surrounding the injured femoral arteries and epididymal adipose tissue at 25 days after arterial injury were collected from HFD-fed or LFD-fed mice treated with vehicle or luseogliflozin. a Representative images of H&E-stained cross-sections of femoral PVAT. b Average adipocyte size of PVAT. c–e Gene expression of adiponectin (c) and Pdgf-b (d), Pdgf receptor-b (e) in femoral PVAT of HFD-fed mice. f Correlation between Pdgf-b expression levels in femoral PVAT and I/M ratio of corresponding femoral artery. White dots, vehicle; back dots, luseogliflozin. g Representative images of immunofluorescence staining for F4/80 in femoral PVAT of HFD-fed mice. h Number of F4/80-positive cells in PVAT. i Representative images of immunofluorescence staining for F4/80 and PDGF-B in femoral PVAT of HFD-fed mice. Upper images, vehicle; lower images, luseogliflozin. j Number of cells F4/80- and PDGF-B-positive cells. k Relative gene expression levels of Il-1b, Il-6, Mcp-1, and Tnf-α in femoral PVAT of HFD-fed mice. White dots, vehicle; back dots, luseogliflozin. l Representative images of H&E-stained cross-sections of epididymal adipose tissue. m Average adipocyte size of epididymal adipose tissue. a, g, i, l Scale bar = 200 μm. bf, k, m: HFD and vehicle, N = 12; HFD and Luseogliflozin (Luseo), N = 8; LFD and vehicle, N = 5; LFD and Luseo, N = 5. h, j Vehicle, N = 4; Luseo, N = 4. *p < 0.05, **p < 0.01

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