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Fig. 5 | Cardiovascular Diabetology

Fig. 5

From: Impaired branched chain amino acid oxidation contributes to cardiac insulin resistance in heart failure

Fig. 5

Improved %EF and unaltered cardiac hypertrophy in TAC mouse hearts post chronic treatment with BT2. Schematic drawing of the protocol for TAC surgery along with BT2 treatment (40 mg/kg/day), a GTT was conducted followed by 6 h fasting (2 g/kg body weight) (a). Representative blots of P-BCKDH, total BCKDH and BCKDH with VDAC as a loading control (b). Levels of cardiac BCAAs (n = 6/group) (c). Densitometry analysis of P-BCKDH normalized to total BCKDH (d), and BCKDK normalized to VDAC (n = 6/group) (e). Changes in ejection fraction (%) (f). Changes in left ventricular mass (g), and posterior wall thickness in diastole (h) and systole (i), as well as peak pressure gradient (j) at 4 week post daily BT2 treatment (n = 9–14/group). Data are presented as mean ± SEM. Data were analysed by using one-way ANOVA with multiple comparison. * or #, as p < 0.05 was considered statistically significant

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