Fig. 4From: Impaired branched chain amino acid oxidation contributes to cardiac insulin resistance in heart failureAccumulation of cardiac BCAAs in TAC mouse hearts. BT2 increases cardiac BCAA oxidation in mouse. Levels of cardiac BCAAs (n = 5/group) (a). Densitometry analysis of BCATm protein expression normalized to Tubulin (n = 6/group) (b). Schematic drawing of BT2 inhibition on BCAA catabolic pathway (c). Rates of BCAA oxidation in normal hearts perfused with BT2 (200 µM) (n = 4/group) (d). Rates of BCAA oxidation in normal hearts at 3 week post daily BT2 treatment (40 mg/kg/day) (Vehicle, n = 6; BT2 Inhibitor, n =) (e). Cardiac work for acute and chronic BT2 treatment was measured as a functional parameter during ex vivo working heart perfusion f and g respectively. Data are presented as mean ± SEM. Data were analysed by t-test. *p < 0.05 was considered statistically significantBack to article page