Fig. 2

Elevated BCAAs and impaired BCAA catabolic pathway in the hearts of patients with DCM. Schematic drawing of BCAA catabolic pathway (a). Levels of cardiac BCAAs (NFC, n = 7; DCM, n = 14) (b). Representative blots of proteins of the BCAA catabolic pathway (c). Densitometry analysis of BCATm normalized to Tubulin (d). Densitometry analysis of P-BCKDH (e), and BCKDH (f), normalized to Tubulin, respectively. Ratio of P-BCKDH ^{(Ser 293)} over the total BCKDH (g). Densitometry analysis of PP2Cm (h), BCKDK (i) and KLF15 (j), normalized to Tubulin, respectively. Densitometry analysis of P-p38MAPK (k) and P-TAK1 ^(thr187) (l), normalized to Tubulin, respectively (n = 6/group). Data are presented as mean ± SEM. Data were analysed by t-test. *p < 0.05 was considered statistically significant