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Fig. 4 | Cardiovascular Diabetology

Fig. 4

From: Early dysregulation of cardiac-specific microRNA-208a is linked to maladaptive cardiac remodelling in diabetic myocardium

Fig. 4

Inhibition of miR-208a ameliorates cardiac hypertrophy in high glucose treated HL-1 mouse cardiomyocytes. a, b: Quantitative scatter plot with a bar graph showing the expression of miR-208a by RT-PCR analysis in normal and high glucose treated HL-1 cardiomyocytes at 24 and 48 h (a) and 24 h after transfection with miR-208a mimic (b). Data are represented as mean ± SEM. ****P < 0.0001 vs. normal glucose (NG) treated cells (a) or scrambled treated cells (b). c, d: Representative fluorescence microscopic images and quantitative scatter plot with a bar graph showing the changes in cardiomyocytes size after treatment with miR-208a mimic (c) or anti-miR-208a (d). Data are represented as mean ± SEM. ****P < 0.0001 vs. scrambled treated group and δδδδP < 0.0001 vs. high glucose (HG)-scrambled sequence treated group. e, f: Representative immunoblots and quantitative bar graphs showing the expression level of α-MHC (e) and β-MHC (f) protein expression in HL-1 cardiomyocytes treated with normal or high glucose and transfected with either scrambled or anti-miR-208a. Data are Mean ± SEM. *P < 0.05 vs. NG-scrambled treated cells and δP < 0.05 vs. HG-scrambled treated cells. Two-way ANOVA was used for comparisons between groups with different treatments, followed by Tukey’s test for multiple comparisons (a, d, e, f) or unpaired student T-Test (b, c). ****P < 0.0001 vs. age-matched non-diabetic control

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