Fig. 3
LV hypertrophy and remodelling of the diabetic heart. a Quantitative line graphs showing the expression of miR-208a by RT-PCR analysis in the myocardium of type 2 diabetic and the age-matched non-diabetic mice at different time points. **P < 0.01, ***P < 0.001 and ****P<0.0001 vs. non-diabetic heart of the corresponding age group. b Scatter plots showing the Pearson correlation coefficient of LV mass vs. miR-208a in all the study animals irrespective of the age or diabetes. There was a significant positive correlation (r = 0.351, P = 0.0002) between LV mass and miR-208a. c–e Representative immunoblots and quantitative bar graphs showing the expression level of α-MHC (c), β-MHC (d) and TR-α (e) protein expression in type-2 diabetic mouse heart collected at 8, 16 and 32 weeks of age. Data are Mean ± SEM and n = 6 samples in each group. Two-way ANOVA was used for comparisons between groups at different time points in mice heart, followed by Tukey’s test for multiple comparisons. ****P < 0.0001 vs. age-matched non-diabetic control