Fig. 8

mAb A treatment does not affect the acetylation of the main energy metabolism regulatory kinases and enzymes post-MI. Overall and specific proteins acetylation levels were measured using immunoprecipitation samples. a Representative blots for total protein acetylation (acetyl-lysine) for molecular weight range of 250–25 kDa. b Representative blots for acetylated-pyruvate dehydrogenase (PDH), acetylated- β-hydroxyacyl CoA dehydrogenase (β-HAD), acetylated-long chain acyl CoA dehydrogenase (LCAD), acetylated-hydroxybutyrate dehydrogenase-1 (BDH-1), acetylated-succinyl-CoA-3-oxaloacid CoA transferase (SCOT) with correspondent α-tubulin as a loading control. Densitometric analysis of c total acetyl-lysine, d acetylated-PDH/α-tubulin, e acetylated-β-HAD/α-tubulin, f acetylated-LCAD/α-tubulin, g acetylated BDH-1/α-tubulin and h acetylated-SCOT/α-tubulin were carried out when protein bands were normalised to α-tubulin bands for intra-experiment variation. Data are reported as mean ± SEM n = 6/group. −C negative control, +C positive control, S sham, V vehicle, Ab mAb A, MI myocardial infarction