Fig. 6

Confocal imaging of intracellular levels of free Na⁺ and H⁺ and analysis of mitochondrial function in DAPA or INSU treated MetS rats compared to MetS rats and age-matched controls. The level of free Na⁺ ([Na]_i) estimated in cells loaded with Na⁺-sensitive SBFI florescent dye (a) and free H⁺ ([H⁺]_i or [pH]_i) with SNARF-1AM (b). The mitochondrial membrane potential (MMP, as FCCP responses) in isolated JC-1 loaded cardiomyocytes (c). The expression levels of mitofusion proteins Mfn-1 and Mfn-2 and their ratio (d). The expression level of mitofission protein Fis-1 (e). The total number of cells/group/protocol; n = 10–19. Significance level at *p < 0.05 vs. CON group and ^#p < 0.05 vs. MetS group