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Fig. 4 | Cardiovascular Diabetology

Fig. 4

From: Regulation of diabetic cardiomyopathy by caloric restriction is mediated by intracellular signaling pathways involving ‘SIRT1 and PGC-1α’

Fig. 4

SIRT1 is required for the expression of PGC-1α. Neonatal Rat cardiomyocytes were exposed to 17.5 and 33 mM glucose and treated with the SIRT1 inhibitor EX-527. Cells were stained with DCF-DA (AD) and fluorescence was measured using fluorimeter (E). Representative western blots for SIRT1 and PGC-1α for cell treated with EX-527 (F). Densitometry analysis for SIRT1 (G) and PGC-1α (H). Results were normalized to the group exposed to 17.5 mM glucose. *p < 0.04 vs.17.5 mM control, &p < 0.02 vs. 33 mM control, n = 4 in each group. Values represent mean ± SD

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