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Fig. 1 | Cardiovascular Diabetology

Fig. 1

From: Glucose-sensing microRNA-21 disrupts ROS homeostasis and impairs antioxidant responses in cellular glucose variability

Fig. 1

miR-21 expression in HUVECs is increased in oscillating (OG) and high stable glucose (HG) conditions. a miR-21 expression was normalised to RNU6 expression using the comparative Ct method. Experiments were performed in duplicate and repeated 6 times. b To assess the relationship between miR-21 and oxidative stress generation, we demonstrated that in 24 h-cultured HUVECs exposed to normal glucose (5 mmol/L) and hydrogen peroxide (H2O2, 500 μM for 6 h), intracellular miR-21 and c extracellular ROS release (measured by EPR instruments); d ROS release in HUVECs, with or without an anti-miR-21 inhibitor, were significantly increased in OG and HG conditions (p < 0.01 and p < 0.001, respectively), demonstrating a potential role for miR-21 in oxidative stress. miR-21 relative expression was normalized to U6 levels. One-way ANOVA followed by Tukey’s post hoc test. *p < 0.05 vs NG, **p < 0.01 vs NG, ****p < 0.0001 vs NG; p < 0.05 vs OG, ††p < 0.01 vs OG; §§§p < 0.001 and §§§§p < 0.0001 vs HG

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