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Fig. 6 | Cardiovascular Diabetology

Fig. 6

From: Adverse effects of Hif1a mutation and maternal diabetes on the offspring heart

Fig. 6

The amount of AGEs is increased whereas the activity of gap junction CX43 is reduced in the heart of diabetes-exposed Hif1a+/ offspring. Representative images of PAS staining of advanced glycation end products (AGE; ad). Scale bar = 50 µm. e–h Delineated PAS+ area in the myocardium by Adobe Photoshop. i Quantification of PAS staining determined as a percentage of positive area in the field of view by ImageJ. The values are mean ± SEM (n = 4). Representative Western blots and quantification of both CX43 and its phosphorylated isoform pCX43 in the LV myocardium of the offspring (j, k). The values are mean ± SEM (n = 3). Statistical significance assessed by two-way ANOVA: diabetes effect (CX43, P = 0.0088) and interaction between genotype and diabetes (AGEs, P = 0.0013; pCX43, P = 0.0030) followed by post hoc Tukey’s multiple-comparison test, *P < 0.05, **P < 0.01, ****P < 0.0001. CX43 connexin 43, PAS periodic acid-shiff, AU arbitrary units

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