Fig. 6From: Adverse effects of Hif1a mutation and maternal diabetes on the offspring heartThe amount of AGEs is increased whereas the activity of gap junction CX43 is reduced in the heart of diabetes-exposed Hif1a+/− offspring. Representative images of PAS staining of advanced glycation end products (AGE; a–d). Scale bar = 50 µm. e–h Delineated PAS+ area in the myocardium by Adobe Photoshop. i Quantification of PAS staining determined as a percentage of positive area in the field of view by ImageJ. The values are mean ± SEM (n = 4). Representative Western blots and quantification of both CX43 and its phosphorylated isoform pCX43 in the LV myocardium of the offspring (j, k). The values are mean ± SEM (n = 3). Statistical significance assessed by two-way ANOVA: diabetes effect (CX43, P = 0.0088) and interaction between genotype and diabetes (AGEs, P = 0.0013; pCX43, P = 0.0030) followed by post hoc Tukey’s multiple-comparison test, *P < 0.05, **P < 0.01, ****P < 0.0001. CX43 connexin 43, PAS periodic acid-shiff, AU arbitrary unitsBack to article page