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Fig. 4 | Cardiovascular Diabetology

Fig. 4

From: Adverse effects of Hif1a mutation and maternal diabetes on the offspring heart

Fig. 4

Maternal diabetes exposure increases apoptosis and Hif1a+/ mutation alters macrophage infiltration in the LV myocardium of the 12-week old offspring. ad Representative images of TUNEL staining (green) with Hoechst stained cell nuclei (blue) in adult myocardium (arrows indicate TUNEL+ cells). Scale bar = 100 µm. e Quantification of TUNEL+ apoptotic cells per mm2 of the LV myocardium. The values are mean ± SEM (n = 4 individuals/3 sections/group). fi Representative confocal images of immunohistochemical staining of macrophage marker F4/80 (green) with Hoechst stained cell nuclei (blue) in adult myocardium (arrows indicate F4/80+ cells). Scale bar = 50 µm. j Quantification of F4/80+ cells per area in the field of view in LV myocardium. The values are mean ± SEM (n = 1 section/2–5 z-stacks/4 individuals/group). k, l Representative Western blot and quantification of TNFR2 expression in the LV myocardium. The values are mean ± SEM (n = 3). Two-way ANOVA indicating interaction between genotype and diabetes (F4/80, P = 0.0002; TUNEL, P = 0.016; TNFR2, P = 0.0133) genotype effect (F4/80, P = 0.0159); diabetes effect (F4/80, P = 0.0042) followed by post hoc Tukey’s multiple-comparison test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. TNFR2 tumor necrosis factor receptor type II, AU arbitrary units

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