Fig. 3

miR-19a reduces the expression of both asTF and flTF in ECs. miR-19a expression in HMEC stimulated with 10 ng/mL TNF-α for 2 or 4 h (a). HMEC were cultured for 24 h and then transfected with a control mimic or miR-19a as well as an inhibitor control or anti-miR-19a. 24 h post transfection the cells were left untreated or stimulated with 10 ng/mL TNF-α. b, d asTF and c, e flTF mRNA under basal conditions and 2 h after stimulation with TNF-α. f Representative western blot shows the protein expression of flTF and asTF upon transfection with miR-19a or anti-miR-19a and their specific controls 6 h post TNF-α stimulation. Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, n ≥ 5