Fig. 5

Effects of linagliptin treatment on latency to fall of wire hang test (a), latency to fall in rotarod test (b), minimum Feret diameter of gastrocnemius muscle (c), renal fibrosis (d), and renal calcification (e) of klotho−/− mice. Abbreviations used are the same as in Fig. 2. NS, not significant. Upper panels in c indicate representative photomicrographs (×400 magnification) of hematoxylin and eosin-stained sections of gastrocnemius muscle from both groups. Upper panels in d, e indicate representative photomicrographs of Sirius red-stained sections and Kossa-stained sections, respectively, of the kidney from both groups of klotho−/− mice. Bar indicates 100 μm in c, d and 500 μm in e. Values are mean ± SEM. n = 7 in each group. In a, b, d, e statistical analysis was performed by non-paired t test. In c statistical analysis was performed by Mann–Whitney U test