Fig. 4

Treatment of MΦ0 WT BMDMs with liraglutide. BMDMs were taken from WT mice and differentiated into MΦ0 macrophages using 25% L929 conditioned medium. MΦ0 macrophages were treated with 1 μg/ml liraglutide ex vivo for 6 h and polarized into MΦ1 (100 ng/ml LPS and 20 ng/ml IFN-gamma) and MΦ2 (20 ng/ml IL-4 and 20 ng/ml IL-13) macrophages for 18 h. Supernatants were analysed by ELISA for a IL-1beta b IL-6 and c IL-10. Cells were analysed by RT-qPCR for d TNF-alpha and e MR. Error bars are representative of a–c four mice (n = 4), d, e three mice (n = 3) each carried out with two replicates. Statistical analysis was carried out performing a Friedman test followed by Dunn’s multiple comparison post-test. *p < 0.05 and **p < 0.01 were considered statistically significant. p > 0.05 was NS. Capped lines indicate comparisons made between groups