Fig. 2
Example of the histograms generated from diabetes clots with added fluorescent markers. (The x-axis represents the grey value range (8-bit greyscale [0, 255]) and the y-axis indicates the number of total pixels for each grey value.) The histograms were generated using FIJI and every composite confocal micrograph contains data of all 3 fluorescent markers. In FIJI the channels can be split and analysed separately, using the histogram function. a Amytrackerâ„¢ 480; b Amytrackerâ„¢ 680; c ThT