Skip to main content
Fig. 5 | Cardiovascular Diabetology

Fig. 5

From: High density lipoprotein modulates osteocalcin expression in circulating monocytes: a potential protective mechanism for cardiovascular disease in type 1 diabetes

Fig. 5

Effect of OxLDL, LDL and HDL on OCN and Runx2 gene and protein expression in THP1 cells. mRNA and protein levels were quantified after treatment with 40 μg/ml OxLDL ± 200 μg/ml HDL ± 40μg/ml LDL for 8 and 24 h (RT-PCR) or for 48 h (immunoblot). OCN (a) and Runx2 (b) mRNA levels (n = 3 for each group) did not change after 8 or 24 h incubation with OxLDL, LDL or HDL. Three-way ANOVA for multiple comparison: p > 0.05. c A representative immunoblot is shown for both OCN and Runx2 proteins. The bar graph shows the quantification of the immunoblot for OCN (n = 3 in each group). OCN protein level significantly increased after incubation with OxLDL alone, but not after incubation with OxLDL + HDL. LDL did not affect OCN levels. Three-way ANOVA for multiple comparison: p < 0.001. *p < 0.05 at the Kruskal–Wallis test for pairwise comparisons. Quantification of Runx2 expression did not show significant changes between groups (data not shown). d Nuclear Runx2 protein levels did not change after incubation with OxLDL, HDL or LDL (n = 3 in each group). Three-way ANOVA for multiple comparison: p > 0.05

Back to article page