Fig. 2

The Nrf2 activator dh404 reduces pro-inflammatory and pro-oxidative markers in diabetic Akita mice. mRNA expression levels of a VCAM-1, b ICAM-1, c p65 Rel A subunit of NF-κB, d IL-1β, e Nox1 and f Nox2 determined by qRT-PCR and expressed as fold induction relative to vehicle controls in WT mice. *P < 0.05, **P < 0.01 and ***P < 0.001 as indicated. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 versus Akita + vehicle. g Representative images and quantification (right panel) of VCAM-1 immunostaining in aortas of WT and Akita KO mice treated with vehicle or dh404. *P < 0.05 versus WT + vehicle group and ^##P < 0.01 versus Akita + vehicle group. Data are presented as ±SEM. N = 6–8 per group