Fig. 6

AGE induced Syk phosphorylation in platelets. a Immunoprecipitation-western blot analysis of Syk phosphorylation after a 5-min stimulation of 100 μg/ml AGE with or without 10 μM PP2. Samples were immunoprecipitated with and Syk antibody, then western blotting with anti-phospho tyrosine antibody (PY100). b The graph shows quantification of the P-Syk/Total Syk ratio. Data are presented as fold changes compared to the control (n = 4, mean ± SEM). Statistical analyses were performed by SPSS two-way ANOVA with post hoc test. *p < 0.05 for indicated comparisons