Fig. 2
AGE induced BDNF release from platelets in a dose-dependent manner. After platelet samples (1 × 108 cells/tube) were incubated at 37 °C by stimulations with 0.1% BSA (control), 25, 50 and 100 μg/ml AGE for 5 min, the supernatants and lysates were collected and BDNF contents were measured by ELISA (n = 10). Data were presented as the ratio of released BDNF/(released BDNF + BDNF content) (mean ± SEM). Statistical analysis was performed by one-way ANOVA. *p < 0.05 vs. control