Skip to main content
Fig. 3 | Cardiovascular Diabetology

Fig. 3

From: Obesity-induced cardiac lipid accumulation in adult mice is modulated by G protein-coupled receptor kinase 2 levels

Fig. 3

Intracellular lipid accumulation in the heart is enhanced in WT compared with GRK2+/− littermates after standard or long-term HFD feeding. a Frozen heart sections were stained with Oil red O and DAPI to visualize and quantify intracellular lipid droplets and nuclei, respectively. Lipid droplets in each condition are shown in black and white after image processing. Results are from at least ten photomicrographs from each heart (magnification ×100). b Percentage of the area occupied by lipid droplets in cardiomyocytes for each condition. c Lipid droplets areas were classified according to their sizes as medium (0.5–1 μm2) or large (>1 μm2). The total area of lipid droplets of each size was normalized with the total area of lipid droplets. Statistical analysis was performed using unpaired two-tail Student’s t test. Data are mean ± SEM (N = 3). *P < 0.05, **P < 0.01

Back to article page