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Fig. 2 | Cardiovascular Diabetology

Fig. 2

From: Obesity-induced cardiac lipid accumulation in adult mice is modulated by G protein-coupled receptor kinase 2 levels

Fig. 2

Low levels of GRK2 protect mice from long-term (30 weeks) HFD-induced heart hypertrophy and fibrosis. a Heart weight to tibial length ratio in HFD-fed 9 month-old WT and GRK2+/− mice, compared with their littermates fed with standard diet (N = 6–9 per genotype and condition). b Cardiomyocyte cross-sectional area from each genotype expressed in μm2 (N = 4). c The ratio of interstitial fibrosis or collagen deposition to the total tissue area was measured in Picro-sirius red-stained sections and expressed as %CVF (collagen volume fraction) (N = 4–7 animals per genotype and condition). Results are from 10 to 15 photomicrographs from each heart (magnification ×40). d Expression of markers of cardiac hypertrophy (Acta1), cardiac stress (BNP) and cardiac functionality (Serca2) were quantified by qPCR and normalized by a geometrical mean of HPRT and RPS29 (N = 5–6). Data are mean ± SEM with a two-tail unpaired Student’s t test statistical analysis.*P < 0.01, ***P < 0.001

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