Fig. 6

Monocyte to macrophage differentiation and lipid uptake in human monocytes isolated from blood. a Representative images of western blot analysis of protein level expression of cyclophilin A, CD 36 and LOX 1 in glucose activated human monocytes during foam cell formation. Protein densities of immunoreactive bands measured by the Quantity One 1D analysis software program. Data are presented as mean ± SD (n = 3) and asterisk represents p < 0.05. b Confocal images of Dil-oxLDL uptake in human monocyte cells differentiated to macrophages. Macrophages were incubated with DiI-ox LDL and acetylated LDL (10 μg/mL for 4 h) before and after treatment with cyclophilin A inhibitor or siRNA and examined using a 559 nm filter. Dil-oxLDL uptake is shown in red and counterstained with Dapi (blue). Mean intensity was quantified using microscope imaging software NIS-Elements Viewer, *p < 0.05 was considered significant