Fig. 6

LDH release and 15-F2t-IsoP levels in cultured cardiomyocytes and H9C2 cells subjected to HG stimulation and hypoxia/reoxygenation (H/R) insult. Isolated cardiomyocytes from non-diabetic rats were treated with or without N-acetylcysteine (NAC, 1 mmol/L) during LG (5.5 mmol/L) or HG (25 mmol/L) conditions for 36 h, or H9C2 cells were transfected with Cav-3 siRNA or eNOS siRNA and treated with or without NAC during LG and HG conditions, then exposed to 4 h of hypoxia followed by 4 h of reoxygenation. a–d LDH release (a) and 15-F2t-IsoP (b) in cultured medium of cardiomyocytes, and LDH release (c) and 15-F2t-IsoP (d) in cultured medium of H9C2 cells. All the results are expressed as mean ± SD, n = 7. Differences were determined by using two-way repeated-measures ANOVA followed by Bonferroni’s post hoc test. *P < 0.05 vs. the other groups in normoxia or H/R conditions