Fig. 4

Expression of p-eNOS and Cav-3 in cultured cardiomyocytes and H9C2 cells after various treatment in LG (5.5 mmol/L) and HG (25 mmol/L) conditions. a Representative western blot of p-eNOS (Ser¹¹⁷⁷) in comparison with total eNOS expression in cardiomyoytes exposed to HG in the presence of N-acetylcysteine (NAC, 1 mmol/L) or methyl-b-cyclodextrin (CD, 10 μmol/L); b–e Representative western blot of Cav-3 (b) and p-eNOS (Ser¹¹⁷⁷) (c), and levels of O₂ ⁻ (d) and NO (e) in H9C2 cells transfected with Cav-3 siRNA or treated with NAC (1 mmol/L). All the results are expressed as mean ± SD, n = 7. Differences were determined by using one-way ANOVA followed by Tukey’s test. ^#P < 0.05 vs. corresponding group without siRNA treatment, *P < 0.05 vs. all the other groups