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Fig. 3 | Cardiovascular Diabetology

Fig. 3

From: N-acetylcysteine attenuates myocardial dysfunction and postischemic injury by restoring caveolin-3/eNOS signaling in diabetic rats

Fig. 3

Expression of Cav-3 and p-eNOS in diabetic hearts, and their association in isolated cardiomyocytes. a, b Representative western blot of Cav-3 expression with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as loading control (a) and p-eNOS expression compared with total eNOS expression (b) in myocardium from the control (c), diabetic (d) and N-acetylcysteine-treated (1.5 g/kg/day, NAC) rats. c Confocal laser microscopic image of isolated cardiomyocytes from non-diabetic rats underwent standard immunofluorescence staining with Cav-3 and eNOS. d The lysates of isolated rat cardiomyocytes from various groups containing equal amount of total protein were subjected to immunoprecipitation (IP) with Cav-3 antibody (Ab) and analyzed by immunoblot (IB) with eNOS and Cav-3 antibody. All the results are expressed as mean ± SD, n = 7. Differences were determined by using one-way ANOVA followed by Tukey’s test. *P < 0.05 vs. all the other groups

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