Fig. 3
Association between ANGPTL3 levels and HDL function in non-diabetic and T2DM female subjects. HDL was isolated from plasma of non-diabetic or T2DM female subjects. RAW264.7 macrophages were loaded with fluorescent labeled cholesterol for 16 h and then exposed to 100 μg/ml HDLs for 4 h. Fluorescence intensity in the supernatant and cells were determined to calculate the percentage of cholesterol efflux towards HDLs. The correlation between ANGPTL3 levels and the percentage of cholesterol efflux in female non-diabetic participants (n = 42) or T2DM patients (n = 45) is shown in a and b, respectively. The parallel lines defined the 90 % prediction band